Rice is one of the most important plants, which grows in different provinces located in north, south and western parts of Iran through a high level of flooding surface irrigation systems with low efficiency. On the other hand, suitable irrigation management programming and the awareness of crop coefficients and exactly, the water requirement of different varieties of this crop is essential in different plant stages. This study was conducted to determine the crop coefficients of rice (Amberbo cultivar) during years 2017 and 2018. For this purpose, water balance drainable lysimeters with a diameter and height of 1. 20m and 1. 40m were used in farm and glasshouse condition, separately. In this regard, the Penman-Monteith equation for potential evapotranspiration and water balance equation to measure actual evapotranspiration were used to calculate potential evapotranspiration. Finally, the average values of crop water requirement and Ambarbo rice crop coefficients were determined in non-flooding condition for different initial, development, middle, and end stages in the farm and glasshouse condition as (584. 59 mm, 0. 96, 1. 07, 1. 25, and 0. 95) and (616. 28 mm, 1. 00, 1. 10, 1. 29, and 1. 05), respectively. The results of this research showed that a lot of rice irrigation water could be saved under Non-Flooding Irrigation Conditions. Therefore, with the saved water, more lands will be cultivated and more production, income and jobs will be provided for the villagers.